Table 1.
Transcription and translation of (A) ano, (B) ECs2384, and (C) ECs2385.
| Condition | RPKM transcriptome∗ | RPKM translatome∗ | Ribosomal coverage value (RCV)∗ | ORF coverage∗ |
|---|---|---|---|---|
| (A) ano | ||||
| LB, 37°C | 50 | 923 | 18.18 | 87% |
| BHI, 37°C | 95 | 261 | 2.72 | 90% |
| BHI + 4% NaCl, 14°C | 46 | 16 | 0.33 | 58% |
| (B) ECs2384 | ||||
| LB, 37°C | 39792 | 68550 | 1.83 | 100% |
| BHI, 37°C | 16006 | 16979 | 1.06 | 100% |
| BHI + 4% NaCl, 14°C | 10197 | 2892 | 0.32 | 100% |
| (C) ECs2385 | ||||
| LB, 37°C | 16 | 21 | 1.42 | 58% |
| BHI, 37°C | 13 | 8 | 0.73 | 56% |
| BHI + 4% NaCl, 14°C | 49 | 7 | 0.15 | 61% |
The RPKM values of the transcriptome and translatome data for the novel gene, the overlapping annotated gene and the upstream-annotated gene are listed. The ribosomal coverage value, a measure of the translatability, was calculated by the ratio of RPKM translatome to RPKM transcriptome. ORF coverage gives the percentage of gene sequence, which is covered by RIBOseq reads. ∗Mean values of the two biological replicates are shown.