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. 2018 May 14;9:937. doi: 10.3389/fmicb.2018.00937

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Copyright © 2018 Cui, Shi, Zhao, Gu, Wei, Yang, Wen, Chen and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplification curve and standard curve of MiCV. (A): Standard curve (Slope: −3.155, Y-Intercept: 39.202, and Efficiency: 107.447) was analyzed with the ABI7500 software. (B): Specificity of the qPCR. Ten other viral pathogens were used for the specificity test. 1: MiCV HEB15 strain DNA; 2–11: DNA and RNA samples of AMDV, MEV, CDV, PRV, MCV, CAV2, RV, PCV1, PCV2, DogCV, and H₂O. (C): Amplification curves. Ten-fold dilutions of standard DNA ranging from 10⁸ copies/μL to 10¹ copies/μL were used as standard controls.