Table 1.
Intra- and inter-assay reproducibility of qPCR.
DNA standard (copies/μL) | Intra-assay reproducibility | Inter-assay reproducibility | ||
---|---|---|---|---|
Mean Ct ± SD | CV (%) | Mean Ct ± SD | CV (%) | |
107 | 16.07 ± 0.25 | 1.58 | 16.53 ± 0.21 | 1.29 |
105 | 22.48 ± 0.38 | 1.69 | 22.92 ± 0.50 | 2.18 |
103 | 28.98 ± 0.21 | 0.73 | 29.28 ± 0.28 | 0.97 |
102 | 31.33 ± 0.25 | 0.79 | 31.82 ± 0.63 | 1.98 |