Table 1.
Antigen | Species | Applications | References | Source |
---|---|---|---|---|
Phosphotyrosine | Mouse | IP (1:360) | 4G10, 05-321 | Millipore |
Actin | Mouse | WB (1:5,000) | A5441 | Sigma Aldrich |
GAPDH | Mouse | WB (1:15,000) | sc-32233 | Santa Cruz Biotechnologies |
K8 | Mouse | WB (1:450), IF (1:200) | sc-8020 | Santa Cruz Biotechnologies |
K8 | Rabbit | WB (1:10,000), IF (1:400) | ab53280 | Abcam |
K18 | Mouse | WB (1:2,000), IF (1:200) | sc-6259 | Santa Cruz Biotechnologies |
K18 | Rabbit | WB (1:10,000), IF (1:400) | ab52948 | Abcam |
cMet | Rabbit | WB (1:175), IF (1:150) | Sc-10 | Santa Cruz Biotechnologies |
TfR | Mouse | WB (1:1500) | 13-6800 | Thermo |
Integrin-β1 | Rabbit | WB (1:1,000) | ab52971 | Abcam |
PI3Kp85 | Rabbit | WB (1:1500) | 06-195 | Millipore |
e-cadherin | Rabbit | WB (1:300) | sc-7870 | Santa Cruz Biotechnologies |
S6 | Mouse | WB (1:1,600) | 2317 | Cell Signaling |
Phospho-S6 | Rabbit | WB (1:1,000) | 4856 | Cell Signaling |
Akt | Rabbit | WB (1:1,000) | 4685 | Cell Signaling |
P-Akt (S473) | Rabbit | WB (1:1,500) | 4060 | Cell Signaling |
WB, Western blot; IF, immunofluorescence.