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Deep RNA sequencing validation through quantitative real time-PCR (qRT-PCR). Validation of 10 representative gene transcripts was performed by qRT-PCR, showing that the behaviour of five up- and five down-regulated genes was consistent in both techniques. log2 fold change (corresponding to −ΔΔCT for qRT-PCR) obtained comparing samples treated with Neuregulin1 (NRG1) with mock treated samples is shown, both for deep sequencing, both for qRT-PCR data.
