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. 2018 May 14;9:1059. doi: 10.3389/fimmu.2018.01059

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Copyright © 2018 Sutoh, Mohamed and Kasahara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree of the SKINT1 subfamily. SKINT1 subfamily sequences were retrieved from the NCBI database (WGS and nr databases) using the mouse Skint1 sequence as a query. Deduced amino acid sequences of SKINT1 subfamily proteins were aligned, and the phylogenetic tree was constructed using the MUSCLE and neighbor-joining programs implemented in MEGA7, respectively. The distance matrix was obtained by calculating p-distances for all pairs of sequences. Gaps were excluded using the pairwise-deletion option. The reliability of branching patterns was assessed by bootstrap analysis (1,000 replications). Pseudogenes are indicated by φ and shown in red. Nodes supported by bootstrap values over 80% are indicated by open circles. Skint7 to Skint11 genes of mice are not shown as they are the members of the SKINT7 or SKINT9 subfamily.

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