Figure 1.

Experimental overview and workflow for transcriptome data normalization, filtering, and analysis. Bone marrow derived macrophages (BMDM) were differentiated for 7 days in L-cell conditioned media, washed and rested for 24 h, prior to acute stimulation with one of four toll-like receptor ligands. Cells were washed after 24 h, rested for 1 h, prior to restimulation with the same ligand. RNA was collected as indicated, from naïve, 4 h (acute) and 4 h (restimulated) time points. Microarray profiling followed, with the numbers of microarray probes, and correspondingly annotated genes that met the criteria for acute induction, or tolerance, as indicated in the table.