Figure 7.

(A) Log₂ expression of two kinases central to the canonical (left) and non-canonical (right) NF-κB pathways. (B) Key components of the canonical and non-canonical NF-κB pathways exhibit different regulatory patterns. Y-axis: Log₂ expression. *indicates significant differential expression (adjusted p < 0.05). (C) StringDB protein–protein association network for the proteins encoded by the 70 genes commonly induced during acute responses by the four toll-like receptor ligands. Nodes: proteins. Edges protein–protein association. Colors: yellow: tolerized; red: super-induced; gray: no memory pattern. (D) Western Blot analysis of p105, p50, and BCL3 expression in cells stimulated for 24 h with ligand for TLR4, TLR2, TLR9, and TLR3 as indicated. (E) Characterization of differential expression and memory status of NF-κB target genes. Figure 7E overlap between NF-κB target genes and differentially expressed genes (adjusted p < 0.05, any acute infection). Number of genes per pattern is indicated in each colored circle. Circle overlaps show numbers of genes with both patterns, either due to multiple differently behaving probes, or ligand-specific memory pattern differences.