Table 2.
Effects of selenium (Se) species supplied on Se speciation in protease XIV extracts from wheat (Triticum aestivum L.) roots and shoots.
| Species | Se species supplied | ||||||
|---|---|---|---|---|---|---|---|
| Selenite | CheSeNPs | BioSeNPs | |||||
| μg g−1 DW | Percentage %* | μg g−1 DW | Percentage % | μg g−1 DW | Percentage % | ||
| Shoot | SeCys2 | 0.33 ± 0.06b | 3.0 ± 0.6 | 1.48 ± 0.06a | 11.4 ± 0.6 | 0.08 ± 0.01b | 2.3 ± 0.1 |
| MeSeCys | 0.24 ± 0.08b | 2.2 ± 0.8 | 0.28 ± 0.05c | 2.2 ± 0.4 | 0.07 ± 0.04b | 2.0 ± 0.5 | |
| Se (IV) | 0.22 ± 0.15b | 2.0 ± 1.6 | 0.38 ± 0.01c | 2.9 ± 0.1 | 0.06 ± 0.02b | 1.7 ± 0.3 | |
| SeMet | 2.05 ± 0.73a | 18.4 ± 0.7 | 1.01 ± 0.11b | 7.8 ± 1.0 | 0.19 ± 0.04a | 5.4 ± 0.6 | |
| Se (VI) | 0.25 ± 0.04b | 2.2 ± 0.5 | 0.22 ± 0.00c | 1.7 ± 0.1 | 0.26 ± 0.01a | 7.4 ± 0.1 | |
| Root | SeCys2 | 2.36 ± 1.09ab | 8.0 ± 2.7 | 2.12 ± 0.13b | 6.6 ± 2.1 | 0.11 ± 0.03b | 0.8 ± 0.1 |
| MeSeCys | 0.90 ± 0.21ab | 3.0 ± 0.5 | 0.85 ± 0.04c | 2.6 ± 0.9 | 0.32 ± 0.07b | 2.4 ± 0.3 | |
| Se (IV) | 3.55 ± 1.02ab | 11.7 ± 2.5 | 11.00 ± 0.15a | 34.0 ± 6.8 | 2.36 ± 1.00a | 17.6 ± 3.6 | |
| SeMet | 5.45 ± 2.27a | 18.0 ± 5.5 | 1.87 ± 0.13b | 5.8 ± 1.7 | 0.67 ± 0.22b | 5.0 ± 0.8 | |
| Se (VI) | 0.40 ± 0.10b | 1.3 ± 0.2 | 0.33 ± 0.06d | 1.0 ± 0.4 | 0.34 ± 0.14b | 2.5 ± 0.5 | |
Mean values (n = 3) ± standard error (SE). Selenite, sodium selenite; CheSeNPs, chemogenic selenium nanoparticels; BioSeNPs, biogenic elemental selenium nanoparticles; SeCys2, selenocystine; MeSeCys, Se-methyl-selenocysteine; Se (IV), selenite; SeMet, selenomethionine; Se (VI), selenate.
*
The percentage was calculated by the concentration of Se species / the concentration of Se in tissues × 100%. The different letters indicate statistically significant differences between the treatments as the p < 0.05.