Figure 3.

A) Relative miR-193b (left) and miR-31 (right) expression compared to the untreated cells. The results normalized by miR-191 for Pfaffl method. Data represent means±SD of three separate tests (*P<0.05). B) qRT-PCR of urokinase-type plasminogen activator (uPA) and Ras homolog gene family member A (RhoA) expression in single miR transfection of MDA-MB231 cell line. C) miR binding site and seed sequence of miR-193b and miR-31. D) Relative expression of the genes compared to the untreated cells. The results normalized by β-actin as the housekeeping gene for Pfaffl method (*P<0.05)