Table 1.
Macro- and microheterogeneity of hFSH preparations.
| Macroheterogeneity (% relative abundance) |
|||||||
|---|---|---|---|---|---|---|---|
| FSH preparation | Pituitary hFSH | Urinary hFSH | Pituitary hFSH24 | Pituitary hFSH21 | Pituitary hFSH21/18 | Recombinant GH3 hFSH24 | Recombinant GH3 hFSH21 |
| FSH24 | 77 | 86 | 100 | – | – | 89 | – |
| FSH21 | 23 | 14 | – | 100 | 60 | 11 | 54 |
| FSH18 | –a | – | – | – | 40 | – | 46 |
| FSH15 | – | – | – | – | – | – | – |
|
Types of oligosaccharides (% relative abundance) |
|||||||
| Oligosaccharide type | Recombinant GH3-hFSHb | ||||||
| Biantennary | 38.2 | 37.2 | 47.1 | 51.2 | 28.6 | 55.5 | |
| Triantennary(3)c | 41.0 | 44.0 | 30.7 | 35.9 | 2.5 | 0 | |
| Triantennary(6)d | 0 | 0 | 0 | 0 | 0 | 29.7 | |
| Tetra-antennary | 15.0 | 14.8 | 10.6 | 6.0 | 0.01 | 0 | |
| Neutral | 0.3 | 2.2 | 9.9 | 4.5 | 74.2 | 12.3 | |
| Sialylated | 99.1 | 97.5 | 75.4 | 78.8 | 20.7 | 87.7 | |
| Sulfated | 6.5 | 4.2 | 39.3 | 35.0 | 9.6 | 0 | |
| Sial/sulfat | 5.9 | 3.9 | 24.0 | 18.3 | 4.5 | 0 | |
| Core fucose | 43.0 | 23.9 | 45.1 | 47.8 | 23.0 | 50.6 | |
| Antenna-fucose | 0.3 | 0 | 3.6 | 0.8 | 0.4 | 19.9 | |
| Bisect GlcNAc | 32.6 | 23.9 | 17.9 | 23.2 | 7.9 | 47.0 | |
| GalNAc | 2.8 | 1.7 | 20.3 | 13.8 | 14.1 | 10.5 | |
Relative abundance determined by Western blot and mass spectrometry, respectively. Data are limited to those preparations for which both glycoform abundance and glycan microheterogeneity exist.
a– = not detected.
b = glycoforms not separated.
cTriantennary(3) = third branch attached to Man(α1–3) branch.
dTriantennary(6) = third branch attached to Man(α1–6) branch.