Table 9. Optimum medium composition and the results of the validation experiment.
Component (%) | MRS medium | Optimum medium |
---|---|---|
Soy peptone | -a | 0.231 |
Proteose peptone | 1.0 | - |
Glucose | 2.0 | 1.232 |
Yeast extract | 0.5 | 1.97 |
Magnesium sulfate | 0.01 | 0.08 |
Ammonium citrate | 0.2 | - |
Beef extract | 1.0 | - |
Manganese sulfate | 0.005 | - |
Potassium phosphate dibasic | 0.2 | - |
Sodium acetate | 0.5 | - |
Tween 80 | 0.1 | - |
Viable cells (Log CFU/mL) | 9.51 | 9.15 |
a absence of constituent.