TABLE 1.
Estimated recoveries of carbon and reducing equivalents from anoxic microcosms of L. terrestris gut contents supplemented with S. cerevisiae lysate, protein, or RNAa
| Product | Recoveries (%)b |
|||||
|---|---|---|---|---|---|---|
| Lysate treatment |
Protein treatment |
RNA treatment |
||||
| Carbon | Reducing equivalents | Carbon | Reducing equivalents | Carbon | Reducing equivalents | |
| CO2 | 5.2 | NAc | 3.1 | NA | 6.9 | NA |
| H2 | NA | 0.7 | NA | 0.1 | NA | 3.1 |
| Acetate | 20.2 | 20.1 | 15.0 | 14.5 | 8.9 | 11.5 |
| Methyl butyrate | 18.1 | 23.4 | 13.8 | 17.4 | —d | — |
| Succinate | 9.0 | 7.8 | — | — | 4.7 | 5.3 |
| Propionate | 0.8 | 0.9 | 4.8 | 5.4 | 0.3 | 0.4 |
| Butyrate | 1.9 | 2.4 | 4.0 | 4.9 | — | — |
| Formate | — | — | — | — | 1.1 | 0.7 |
| Lactate | 0.1 | 0.1 | — | — | 1.7 | 2.2 |
| Total | 55.3 | 55.4 | 40.4 | 42.1 | 23.6 (44.9)e | 23.2 (34.1)e |
a
See Fig. 1 for product profiles of lysate and Fig. 3 for product profiles of protein and RNA treatments. The method for calculating recoveries is provided in Materials and Methods.
b
Recoveries were calculated at the end of the 30-h incubation and are based on the arithmetic average from three replicate analyses.
c
NA, not applicable.
d
—, no net increase of the product during the 30-h incubation.
e
Parenthetical values are the estimated recoveries based on RNA-derived ribose as sole source of carbon and reducing equivalents.