TABLE 1.
Oligonucleotide primers used in this study
| Primer | Sequence (5′–3′)a | Purpose |
|---|---|---|
| YC-BcATG8-F | TCACACTGGCGGCCGCTCGAGATGCGATCCAAGTTTAAGGACG | Amplification of full cDNA sequence of the BcATG8 gene for yeast complementation assays |
| YC-BcATG8-R | CCCTCTAGATGCATGCTCGAGTTAGTTGGCTTCTTCTAAAGCTTCAC | |
| Y2H-BcAtg4-F | GCCATGGAGGCCAGTGAATTCATGACGGCGGCTGATTTAGG | Amplification of full cDNA sequence of the BcATG4 gene for yeast two-hybrid assays |
| Y2H-BcAtg4-R | ATGCCCACCCGGGTGGAATTCTTAGGCGTCTAATATGGTATCGTCA | |
| Y2H-BcAtg8-F | ATGGCCATGGAGGCCGAATTCATGCGATCCAAGTTTAAGGACG | Amplification of full cDNA sequence of the BcATG8 gene for yeast two-hybrid assays |
| Y2H-BcAtg8-R | TCGACGGATCCCCGGGAATTCTTAGTTGGCTTCTTCTAAAGCTTCAC | |
| P1 | GGTGGTAGCAGGACAAGAC | Amplification of BcATG8 upstream fragment for construction of the gene deletion vector |
| P2 | CAAAATAGGCATTGATGTGTTGACCTCCTGTAATGGAGGATGATGGAG | |
| P3 | CTCGTCCGAGGGCAAAGGAATAGAGTAGCGCTTCCTTAGACACTTCAG | Amplification of BcATG8 downstream fragment for construction of the gene deletion vector |
| P4 | ACCATCCTCTTCACTTCTAC | |
| P5 | TATACCTACCTGTCTAGAGAC | Amplification of the BcATG8 gene deletion vector |
| P6 | AGCTACTCAAGTCATGATTC | |
| P7 | TCAGCATCAGAATAAGCATC | Identification of BcATG8 deletion transformants |
| P8 | TCCAAAGAGCATACACAACT | |
| HPH-F | GGAGGTCAACACATCAATGCCTATT | |
| HPH-R | CTACTCTATTCCTTTGCCCT | Amplification of the hygromycin resistance gene, HPH |
| BcATG8-C-F | ccggaattccggGAGCGAGAGCGAGAGAAGTC | Amplification of full-length BcATG8, including 1,116-bp upstream and 92-bp downstream fragments, for complementation of the BcATG8 deletion mutant |
| BcATG8-C-R | cgcggatccgcgGTAATGTAGTCACACTGCCC | |
| GFP-BcAtg8-F | CTTGGGAATGGATGAACTTTACAAAATGCGATCCAAGTTTAAGGACG | Amplification of full-length BcATG8 fragment used for construction of the GFP-BcAtg8 vector |
| GFP-BcAtg8-R | TAATCATACATCTTATCTACATACGTTAGTTGGCTTCTTCTAAAGC | |
| YG8-F | CTCGTGCCGAGGTTAAGTTC | Identification of the in-frame GFP-BcAtg8 fusion vector |
| YG8-R | CATCCTCATCCTTATGCTCC | |
| Probe-F | GGTGGTAGCAGGACAAGACT | Amplification of BcATG8 upstream fragment used as the probe for Southern blot analysis |
| Probe-R | TGTAATGGAGGATGATGGAG | |
| A-F | AGCGGAGCAATCGATGTTATAG | Amplification of the BC1G_13009 gene in quantitative real-time PCR assays |
| A-R | TATGGCCGAAGGAGAGAGAA | |
| B-F | GGCTTGCGTCTGTGTCTAA | Amplification of the BC1G_11851 gene in quantitative real-time PCR assays |
| B-R | CGCCTCCAACAACGATAGTAA | |
| C-F | CCTCCGTTCAATCGATGTTCT | Amplification of the BC1G_10262 gene in quantitative real-time PCR assays |
| C-R | GCGAGACTCTTGAGGTGTATTC | |
| D-F | GGGTGAGCAGATCAAGGATATT | Amplification of the BC1G_07580 gene in quantitative real-time PCR assays |
| D-R | GTCAGATGTGGGTGGGATTT | |
| E-F | GGAAAGGGAGGAGTACCAAATC | Amplification of the BC1G_03357 gene in quantitative real-time PCR assays |
| E-R | GGGTTACAGAAGACCCGTAAAG | |
| F-F | CTTCATCCCTCTACCTCGAAATC | Amplification of the BC1G_12236 gene in quantitative real-time PCR assays |
| F-R | CCCTTTCACAACCGCATCTA | |
| G-F | CAGCGTACCCTCATCTGTATTC | Amplification of the BC1G_09602 gene in quantitative real-time PCR assays |
| G-R | GGGCTCGTCATCGTACATTT | |
| H-F | CCCGTCGTCATTCACAAGAA | Amplification of the BC1G_07986 gene in quantitative real-time PCR assays |
| H-R | TAACTCCCGCTCCCATCATA | |
| Actin-F | CGTCACTACCTTCAACTCCATC | Amplification of the actin gene in quantitative real-time PCR assays |
| Actin-R | CGGAGATACCTGGGTACATAGT |
a
Restriction enzyme sites included in primers are in lowercase.