TABLE 3.
Environmental powder and soil samples (NATO SIBCRA exercise)
| Sample no.a | Matrix | Spiking status | Concentrationb | RPA resultsd |
||
|---|---|---|---|---|---|---|
| BA_5345 | lef (pXO1) | capA (pXO2) | ||||
| 1c | DiPel powder | B. thuringiensis | ND | − | − | − |
| 2 | DiPel powder | B. thuringiensis plus B. anthracis Sterne | ND | + | + | − |
| 3c | Backing soda | None | ND | − | − | − |
| 4 | Backing soda | B. anthracis Sterne | ND | + | + | − |
| 5c | Yeast powder | None | ND | − | − | − |
| 6 | Yeast powder | B. anthracis Sterne | ND | + | + | − |
| 7c | Cream powder | None | ND | − | − | − |
| 8 | Cream powder | B. anthracis Sterne | ND | + | + | − |
| 9 | Soil | B. anthracis | 2 × 104 CFU/g | + | + | + |
| 10 | Soil | B. anthracis | 2 × 105 CFU/g | + | + | + |
| 11 | Soil | B. anthracis | 2 × 107 CFU/g | + | + | + |
| 12 | Soil | B. thuringiensis | 10 mg/g | − | − | − |
| 13 | Soil | Vaccinia virus | 2 × 107 CFU/g | − | − | − |
| 14 | Soil | F. tularensis | 2 × 107 CFU/g | − | − | − |
| 15 | Soil | B. pseudomallei | 2 × 107 CFU/g | − | − | − |
| 16 | Soil | F. tularensis plus B. pseudomallei | 2 × 106 CFU/g each | − | − | − |
a
Nucleic acid extracts from these samples were investigated in the frame of SIBCRA exercises. Laboratory results were positive for all agents other than B. anthracis (i.e., vaccinia virus, F. tularensis, B. pseudomallei, and mixed F. tularensis and B. pseudomallei).
b
ND, not determined.
c
To confirm that DNA in the extract was amplifiable, samples were assessed using a 16S rRNA PCR.
d
−, not detected; +, detected.