TABLE 5.

Blind testing at the Institute of Veterinary Bacteriology (Bern, Switzerland)

Strain	Sample type	Speciesa	RPA resultb			Identification
			BA_5345	lef (pXO1)	capA (pXO2)
JF3788	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3786	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3852	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3787	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3785	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3784	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3853	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3854	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3783	DNA extract	B. anthracis	+	+	+	B. anthracis
JF3960	Lysate	B. anthracis	+	+	+	B. anthracis
JF3965	Lysate	B. anthracis	+	+	+	B. anthracis
JF3966	Lysate	B. anthracis	+	+	+	B. anthracis
JF3963	Lysate	B. anthracis	+	+	+	B. anthracis
JF3961	Lysate	B. anthracis	+	+	+	B. anthracis
Chad A1	Lysate	B. anthracis	+	+	+	B. anthracis
Chad A3	Lysate	B. anthracis	+	+	+	B. anthracis
Chad A5	Lysate	B. anthracis	+	+	+	B. anthracis
Chad A7	Lysate	B. anthracis	+	+	+	B. anthracis
Chad A10	Lysate	B. anthracis	+	+	+	B. anthracis
Chad A12	Lysate	B. anthracis	+	+	+	B. anthracis
JF4875c	DNA extract	B. cereus	−	−	−	B. cereus
JF5512c	DNA extract	B. cereus	−	−	−	B. cereus
JF4075c	DNA extract	B. cereus	−	−	−	B. cereus
M2841c	DNA extract	B. cereus	−	−	−	B. cereus
JF5881c	DNA extract	B. cereus	−	−	−	B. cereus
JF3778c	DNA extract	B. cereus	−	−	−	B. cereus
JF1887c	DNA extract	B. cereus	−	−	−	B. cereus
M2089c	DNA extract	B. cereus	−	−	−	B. cereus
JF4090c	DNA extract	B. cereus	−	−	−	B. cereus
JF4059c	DNA extract	B. cereus	−	−	−	B. cereus

^aGenomic DNA was characterized by real-time PCR TaqMan assays targeting sap (chromosomal), cap (pXO2), and pag (pXO1) genes.

^b−, not detected; +, detected.

^cEndpoint PCR of the panC gene confirmed the presence of amplifiable DNA in all negative samples (B. cereus group).