TABLE 2.
Description of the presequencing technical procedures used in this studya
| Type of trap | Spore recovery | DNA extraction | No. of unexposed replicates | No. of exposed replicates | Code |
|---|---|---|---|---|---|
| Whatman no. 1 | Shaking | Macherey-Nagel minikit | 3 | 3 | W1_S_mnM |
| Whatman no. 1 | Shaking | Qiagen minikit | 3 | 3 | W1_S_mnQ |
| Whatman no. 1 | Rubbing | Macherey-Nagel minikit | 3 | 3 | W1_R_mnM |
| Whatman no. 1 | Rubbing | Qiagen minikit | 3 | 3 | W1_R_mnQ |
| Whatman no. 1 | Grinding | Macherey-Nagel maxikit | 2 | 3 | W1_G_mxM |
| Whatman no. 1 | Grinding | Qiagen maxikit | 2 | 3 | W1_G_mxQ |
| Whatman no. 3 | Shaking | Qiagen minikit | 3 | 3 | W3_S_mnQ |
| Whatman no. 3 | Shaking | Macherey-Nagel minikit | 3 | 3 | W3_S_mnM |
| Whatman no. 3 | Rubbing | Qiagen minikit | 3 | 3 | W3_R_mnQ |
| Whatman no. 3 | Rubbing | Macherey-Nagel minikit | 3 | 3 | W3_R_mnM |
| Whatman no. 1 + glue | Rubbing | Macherey-Nagel minikit | 3 | 3 | W1g_R_mnM |
| Whatman no. 1 + glue | Rubbing | Qiagen minikit | 3 | 3 | W1g_R_mnQ |
| Whatman no. 1 + glue | Grinding | Qiagen maxikit | 2 | 3 | W1g_G_mxQ |
| Whatman no. 1 + glue | Grinding | Macherey-Nagel maxikit | 2 | 3 | W1g_G_mxM |
| Whatman no. 3 + glue | Rubbing | Macherey-Nagel minikit | 3 | 3 | W3g_R_mnM |
| Whatman no. 3 + glue | Rubbing | Qiagen minikit | 3 | 3 | W3g_R_mnQ |
| Petroleum jelly + paraffin wax | Grinding | Qiagen minikit | 3 | 3 | PJ_G_mnQ |
| Petroleum jelly + paraffin wax | Grinding | Qiagen maxikit | 2 | 3 | PJ_G_mxQ |
| Petroleum jelly + paraffin wax | Grinding | Macherey-Nagel minikit | 3 | 3 | PJ_G_mnM |
| Petroleum jelly + paraffin wax | Grinding | Macherey-Nagel maxikit | 2 | 3 | PJ_G_mxM |
a
A sample and its replicates were composed of the combination of the type of trap, the spore recovery procedure, and the DNA extraction kit. All unexposed and exposed samples were sequenced with the Illumina MiSeq technology targeting two subloci of the fungal internal transcribed spacers, ITS1 and ITS2.