TABLE 4.
Plasmids used in this study
| Plasmid | Plasmid characteristicsa | Source or reference |
|---|---|---|
| pJET1.2/blunt | Apr ori ColE1 eco47IR, high copy-no. cloning vector | Thermo Fisher Scientific, Lithuania |
| pTZ57R/T | Apr ori ColE1 lacZα, high copy-no. cloning vector | Thermo Fisher Scientific, Lithuania |
| pET-28b | pBR322-derived ColE1, T7 lac promoter, Kmr, protein expression vector | Novagen, Germany |
| pRSFDuet-1 | RSF1030-replicon, T7 lac promoter, two MCSs, Kmr, protein expression vector | Novagen, Germany |
| pCDFDuet-1 | CloDF13 replicon, T7 lac promoter, two MCSs, Smr, protein expression vector | Novagen, Germany |
| pBAD24 | ColE1 ori, Apr, araC promoter, protein expression vector | Invitrogen |
| pBBR1MCS-1 | pBBR1-derived, Cmr, lacZα, broad-host-range vector | 28 |
| pBAD-MCS-1 | Amplicon containing regulatory elements of pBAD24 was amplified by PCR using primers Vsp_F_BAD and Sac_R_BAD, digested with VspI and SacI, and cloned into pBBR1MCS vector | This study |
| HpdF_pET | hpdF gene was amplified by PCR using HpdF_F_Nco and HpdF_R_Hind primers, digested with NcoI and HindIII, and cloned into pET-28b vector | This study |
| HpdABCDE_pET | Monooxygenase gene cluster was amplified by PCR using P1_PciI_F and P5_Hind_R primers, digested with PciI and HindIII, and cloned into pET-28b vector | This study |
| HpdABCDE_pBAD-MCS-1 | pET_ HpdABCDE plasmid was digested with XbaI and HindIII, monooxygenase gene containing fragment was isolated, and cloned into pBAD-MCS-1 vector | This study |
| pART3-gfp | Escherichia coli-Arthrobacter shuttle vector | 40 |
| gfp_pBAD-MCS-1 | pART3-gfp plasmid was digested with XbaI and HindIII, gfp gene containing fragment was isolated and cloned into pBAD-MCS-1 vector | This study |
| gfp_pBBR1MCS-1 | pART3-gfp plasmid was digested with KpnI and HindIII, gfp gene containing fragment was isolated and cloned into pBBR1MCS-1 vector | This study |
| pSal_4 | 2HP degradation genes containing plasmid obtained during transposon mutagenesis | This study |
| pDra_1 | 2HP degradation genes containing plasmid obtained during transposon mutagenesis | This study |
a
Apr, apramycin resistance; Kmr, kanamycin resistance; MCS, membrane contact sites; Smr, streptomycin resistance.