TABLE 1.
Expression of genes encoding putative plant biomass polysaccharide-degrading enzymes during the growth of D. squalens dikaryon FBCC312 in the presence of cellobiose compared to d-glucosea
| Protein ID | Enzyme code | Gene name | Fold change over d-glucose | P value over d-glucose | FPKM cellobiose | Putative substrate | CAZyme familyb |
|---|---|---|---|---|---|---|---|
| 829728 | BGL | 18.7 | 1.32E−75 | 229.2 | Cellulose | GH1 | |
| 1001790 | BGL | 7.2 | 5.90E−30 | 38.5 | Cellulose | CBM1-GH3 | |
| 922530 | BGL | 3.5 | 1.79E−15 | 44.3 | Cellulose | GH3 | |
| 931309 | BGL | 3.0 | 4.16E−20 | 96.5 | Cellulose | GH3 | |
| 944872 | CBH | cel7c | 27.3 | 3.19E−88 | 969.9 | Cellulose | GH7 |
| 918783 | CBH | cel7a | 14.6 | 1.83E−19 | 203.0 | Cellulose | GH7 |
| 803735 | CBH | cel6a | 9.6 | 7.73E−46 | 451.4 | Cellulose | CBM1-GH6 |
| 946952 | CBH | cel7b | 8.6 | 3.96E−11 | 252.4 | Cellulose | GH7 |
| 1015695 | CDH | 12.4 | 6.64E−47 | 107.7 | Cellulose | AA8-AA3_1 | |
| 931204 | EGL | 19.7 | 7.98E−44 | 182.3 | Cellulose | GH131-CBM1 | |
| 830363 | EGL | 18.1 | 6.75E−69 | 199.8 | Cellulose | CBM1-GH5_5 | |
| 955303 | EGL | 9.6 | 1.09E−63 | 426.1 | Cellulose | CBM1-GH5_5 | |
| 946842 | EGL | 8.2 | 5.82E−23 | 52.8 | Cellulose | CBM1-GH5_5 | |
| 991840 | EGL | 5.0 | 3.33E−12 | 140.6 | Cellulose | GH45 | |
| 399056 | LPMO | lpmo2 | 20.3 | 1.86E−56 | 417.2 | Cellulose | AA9 |
| 815832 | LPMO | 14.3 | 5.92E−34 | 99.1 | Cellulose | AA9-CBM1 | |
| 948592 | LPMO | 14.2 | 1.73E−45 | 269.7 | Cellulose | AA9-CBM1 | |
| 465156 | LPMO | 9.1 | 1.09E−33 | 340.1 | Cellulose | AA9 | |
| 816464 | 23.8 | 3.88E−40 | 160.6 | Cellulose | CBM1 | ||
| 1017331 | LPMO | 20.7 | 8.23E−52 | 280.7 | Cellulose/hemicellulose | AA9 | |
| 512221 | LPMO | 18.3 | 3.22E−54 | 417.8 | Cellulose/hemicellulose | AA9 | |
| 928670 | LPMO | 12.0 | 6.85E−26 | 195.7 | Cellulose/xylan | AA9 | |
| 972934 | LPMO | 2.8 | 0.003 | 17.6 | Cellulose/xyloglucan | AA9 | |
| 38831 | EXPN | 2.8 | 0.0001 | 24.5 | Expansin-like | NA | |
| 1040576 | AXE/GMAE | 16.2 | 1.08E−33 | 79.1 | Hemicellulose | CBM1-CE16 | |
| 938389 | AGL | 3.4 | 5.43E−13 | 109.6 | Mannan | GH27 | |
| 825072 | MAN | 3.5 | 8.65E−18 | 82.4 | Mannan | CBM1-GH5_7 | |
| 811661 | MAN | 2.8 | 1.99E−11 | 134.1 | Mannan | GH5_7 | |
| 938021 | BXL | 7.0 | 4.32E−22 | 101.1 | Xylan | GH5_22 | |
| 945667 | XLN | 9.7 | 9.97E−34 | 82.2 | Xylan | CBM1-GH10 | |
| 810461 | XLN | 6.4 | 7.48E−07 | 11.5 | Xylan | CBM1-GH10 | |
| 725679 | XG-EGL | 36.0 | 7.12E−53 | 200.8 | Xyloglucan | GH12 | |
| 805675 | XG-EGL | 13.8 | 5.31E−48 | 243.5 | Xyloglucan | GH74-CBM1 | |
| 417119 | XG-EGL | 3.8 | 8.38E−07 | 67.7 | Xyloglucan | GH12 |
a
Only genes for which expression was at least 2.5-fold higher than on d-glucose with a P value of ≤0.01 and an FPKM value of >10 are included.
b
NA, not applicable.