TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant propertiesa | Reference or source |
|---|---|---|
| Strains | ||
| D. discoideum | ||
| Ax3 | Parental strain | 32 |
| Ax3 ΔDd5P4 | Dd5P4 deletion mutant, Blsr | 32 |
| Ax2 | Parental strain | 71 |
| Ax2 ΔWASH | WASH deletion mutant, Blsr | 71 |
| E. coli TOP10 | Invitrogen | |
| L. pneumophila | ||
| GS3011 | L. pneumophila JR32 icmT3011::Kanr (ΔicmT) | 74 |
| JR32 | Virulent L. pneumophila serogroup 1 strain, derivative of strain Philadelphia-1 | 75 |
| Plasmids | ||
| AmtA-mCherry | pDM1044-amtA-mCherry | 56 |
| pAW1 | pDM323-vps26-gfp | This work |
| pAW2 | pDM323-vps29-gfp | This work |
| pAW3 | pDM323-vps35-gfp | This work |
| pAW5 | pDM323-vps10-gfp | This work |
| pAW7 | pDM317-gfp-rab1A | This work |
| pAW9 | pDM317-gfp-rab7A | 44 |
| pAW10 | pDM317-gfp-rab8A | This work |
| pAW12 | pDM1044-cnxA-mCherry | 53 |
| pAW14 | pMMB207-C-Ptac-mplum, ΔlacIq (constitutive mPlum), Camr | 53 |
| pAW16 | pDM323-cnxA-gfp | 44 |
| pAW18 | pDM317-gfp-vps5 | This work |
| pCR76 | pMMB207-C-Ptac-RBS-gfp-RBS-MCS (constitutive gfp) Camr | 43 |
| pCR110 | pDXA-HC-vps10-gfp | 43 |
| pCR111 | pDXA-HC-vps26-gfp | 43 |
| pCR112 | pDXA-HC-vps29-gfp | 43 |
| pCR113 | pDXA-HC-vps35-gfp | 43 |
| pDM317 | Dictyostelium expression vector, extrachromosomal, N-terminal GFP, G418r | 76 |
| pDM323 | Dictyostelium expression vector, extrachromosomal, C-terminal GFP, G418r | 76 |
| pDM1044 | Dictyostelium expression vector, extrachromosomal, C-terminal mCherry, Hygr | 56 |
| pFL1329 | pDXA-vps5-gfp | This work |
| pNT-28 | pMMB207-C Ptac-gfp ΔlacIq (constitutive gfp) Camr | 77 |
| pSU12 | pDXA-gfp-rab8A | 11 |
| pSU22 | pDXA-gfp-rab1A | 11 |
| pWS22 | pBsrH-P4CSidC-mRFPmars | 16 |
| pWS34 | pDM323-P4CSidC-gfp | This work |
a
Abbreviations: Bls, blasticidin; Cam, chloramphenicol; Hyg, hygromycin; Kan, kanamycin; G418, Geneticin.