TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′–3′)a | Comment |
|---|---|---|
| oAW1 | ATA TAT GGA TCC ATG AAC TTT TTT AGT AAT AAT | 5′ of vps26, BamHI |
| oAW2 | ATA TAT ACT AGT ATC ACT TTC ATC GGA AGA G | 3′ of vps26, SpeI |
| oAW3 | ATA TAT GGA TCC ATG TTT ATT ATT GCA ATT G | 5′ of vps29, BamHI |
| oAW4 | ATA TAT ACT AGT TTG TTG TTT GAC ATG ATC | 3′ of vps29, SpeI |
| oAW5 | ATA TAT GGA TCC ATG ATG AAA AAA AAT AAA CC | 5′ of vps35, BamHI |
| oAW6 | ATA TAT ACT AGT AAT TGA AAT ACC TTT ATA ATT TTG | 3′ of vps35, SpeI |
| oAW9 | ATA TAT GGA TCC ATG AAC CCA GAT TAT C | 5′ of rab1A, BamHI |
| oAW10 | ATA TAT ACT AGT ACA ACA ACC AGA TTT TG | 3′ of rab1A, SpeI |
| oAW15 | ATA TAT GGA TCC ATG ACT TCT CCA GC | 5′ of rab8A, BamHI |
| oAW16 | ATA TAT ACT AGT ACA ACA AGC TTT TTT CTT ATT G | 3′ of rab8A, SpeI |
| oAW19 | ATA TAT GGA TCC ATG TCA GAT TAT AAT AGT AAT C | 5′ of vps5, BamHI |
| oAW20 | ATA TAT ACT AGT AAT AGA AGT TGA ACC CCA AG | 3′ of vps5, SpeI |
| oAW21 | ATA TAT AGA TCT ATG AAG ATT TCA TC | 5′ of vps10, BglII |
| oAW22 | ATA TAT ACT AGT AAA ATC TTC GTT ATC | 3′ of vps10, SpeI |
| oWS17 | AAA AAG ATC TAT GTC AAA ATA TTC CTC C | 5′ of P4CSidC, BglII |
| oWS18 | AAA AACTAGTAA AGA ATT CAA TTG CTT CAT | 3′ of P4CSidC, SpeI |
a
Restriction sites are underlined.