Fig. 2.
THP-1 cells were treated with PMA (“PMA”) or PMA plus hydrocortisone (“PMA + HC”) and then mock infected or hCMV (Towne strain) infected as labeled in rows. In columns, “IE2” indicates staining with primary mouse anti-hCMV IE antigen (IE2) MAb followed by secondary antibody with Alexa Flor 350; “DAPI” indicates live cells; “Merge” indicates live cells expressing hCMV IE antigen that were positive for both DAPI and IE2 staining.