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. 2018 Mar 24;46(9):4546–4559. doi: 10.1093/nar/gky218

Figure 1.

Figure 1.

E2F7 represses the expression of genes involved in cell cycle and DNA repair pathways. (A) Functional classification of E2F7-regulated genes by GSEA analysis using Pathway Interaction Database. The heat map shows the enriched pathways among the list of genes differentially expressed in cells lacking E2F7. Normalized enrichment ratios obtained in GSEA analyses are represented as colors (red for upregulated genes, green for downregulated genes). Only pathways with FDR < 10% were considered significantly enriched. (B) Validation of RNA-seq results. U2OS cells transfected with NT control or E2F7 siRNAs were synchronized in the cell cycle by HU treatment. RT-qPCR analyses of indicated genes were carried out with RNA samples harvested at 0h (G1/S), 3 h (S phase) and 12 h (G2/M) after HU treatment release. Expression values are normalized to the expression of EIF2C2, used as standard control. Data are represented as fold-change (mean ± SEM) relative to siNT-transfected samples from three independent experiments (*, P < 0.05).