Figure 6.

E2F7 suppresses HR through transcriptional repression of DNA repair genes. (A) U2OS-DR-GFP cells were transfected with siRNAs specific for E2F7 or with a plasmid expressing E2F7 together with an SceI expression vector. GFP-positive cells were analyzed by FACS. Data are shown as a percentage over siNT or over empty pCMV transfection. The values shown represent the mean ± SD of three independent experiments (*, P < 0.05). (B) U2OS-DR-GFP cells were transfected with siRNAs specific for E2F7, RAD51 or with a combination of both and analyzed as in (A). Western blot analysis confirms knockdown of E2F7 and RAD51 (arrow). Shown is a representative experiment of two independent experiments. A non-specific band in RAD51 blot is indicated with an asterisk.