Figure 6.

CAF-1 is responsible for Cse4 incorporation at promoters of differentially expressed genes. (A) Growth curves show OD₆₀₀ of WT, psh1Δ, cac2Δ and psh1Δ cac2Δ strains over time after galactose induction of Cse4 overexpression. Overnight cultures grown in raffinose medium were diluted to OD₆₀₀ of 0.1. After 2 h at 30°C, Cse4 overexpression was induced by adding galactose to a final concentration of 4% (t 0) and cultures were grown for 14 h. OD₆₀₀ was measured at each hour after galactose addition. (B) Venn diagrams indicate the number of significantly up- and downregulated genes between psh1Δ, cac2Δ and psh1Δ cac2Δ strains relative to WT (adjusted P < 0.01 and fold change > 1.5). (C) Metagene plots show the mean Cse4-ChIP coverage at promoters of genes downregulated or upregulated in the psh1Δ strain relative to WT. A segment of −500 to +100 bp relative to TSS is shown. The nucleosome free region (NFR, −200 to -100 bp) is indicated.