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. 2018 Apr 2;293(20):7786–7795. doi: 10.1074/jbc.RA117.001513

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© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — MinC and MinD from P. aeruginosa assembled into copolymers with a 1:1 stoichiometry in the presence of ATP. A, MinC and MinD assembled into straight filaments and bundles observed by EM. Bar is 200 nm. B, SDS-PAGE analysis of cosedimentation in different ratios of MinC and MinD showed that the pellet always contained a 1:1 MinC:MinD, and assembly required a minimum of 4–5 μm MinD. S, supernatant; P, pellet. C and D, the concentrations of MinC and MinD in the pellet were calculated through SDS-PAGE analysis. Each value is the average of four independent analyses.