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. 2018 Apr 2;293(20):7786–7795. doi: 10.1074/jbc.RA117.001513

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Figure 2. — Assembly kinetics measured by light-scattering assay. A, assembly kinetics of 10 μm MinC and different concentration of MinD. B, assembly kinetics of 10 μm MinD and different concentration of MinC. C, comparison of the MinC–MinD copolymer assembly kinetics of premixed MinC and MinD proteins or premixed MinD and ATP. Assembly was initiated by adding ATP or MinC, respectively. MinD was 10 μm, MinC was 3 μm, and ATP was 1 mm. D, comparison of the assembly kinetics of 10 μm MinD and low concentrations of MinC (0.5, 1, or 2 μm). Red curves are premixed MinC and MinD, and green curves are premixed MinD and ATP. ATP was 1 mm. E, the fluorescence recorded from 380 to 600 nm of 0.2 μm mant-ATP after adding different concentration of MinD proteins. F, fluorescence at the 445 nm peak plotted against the concentration of MinD (average and standard deviation of two assays). The curve is fitted using a simple binding model (“Experimental Procedures”) giving a K_D of 0.6 μm for the best fit.