Figure 2.

Effect on growth after production of TatA and truncated TatA variants without HiPIP fusion. A, growth curves of the tatAE-deletion strain JARV16 with recombinant rhamnose-induced production of either TatA, TatA-49, TatA-42, TatA-33, or TatA-NT. All constructs were Strep-tagged. Induction of the pBW expression vectors was done with 0.1% rhamnose at the indicated time point. S.D. values (error bars) were calculated from three independent cultures. B, Tricine-SDS-PAGE/Western blot analysis of fractioned cells of JARV16 producing the indicated TatA-NT variants or TatA. S, soluble fraction; M, membrane fraction. C, carbonate washes of TatA and the truncated TatA variants. M, membranes; S, supernatant after carbonate wash; P, pellet fraction after carbonate wash. Western blots were developed using Strep-Tactin-AP conjugate.