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. 2018 Mar 13;293(20):7592–7605. doi: 10.1074/jbc.RA118.002205

Figure 4.

Figure 4.

TatA-NT-Hip reduces the membrane potential. A, fluorescence microscopy of JC1 staining of JARV16 producing either TatA-Hip, TatA-NT-Hip, TatA-NT(LA)6-Hip, or TatA-NT(STGG)3-Hip. All constructs were Strep-tagged. Formation of red J-aggregates was strongly reduced in TatA-NT-Hip– and TatA-NT(LA)6-Hip–producing cells. B, ratio of J-aggregates to JC1 monomers (590 nm/530 nm) of JARV16 producing the indicated TatA-NT-variant or TatA-Hip. Emission spectra were recorded with an excitation of 485 nm and emission from 500 to 650 nm. Ratios of peak maxima at 590 and 530 nm were calculated. Error bars, S.D.