Figure 1.

TIGAR regulates canonical NF-κB signaling. NT, TKD, and TOE 3T3-L1 preadipocytes were generated as described under “Method details.” A, 3T3-L1 adipocytes were either left untreated (Basal) or stimulated with 10 ng/ml TNFα for 5 min (TNFα). Cell lysates were prepared and immunoblotted for the indicated proteins. These are representative immunoblots independently performed five times. B and C, the adipocytes were either left untreated or stimulated with 10 ng/ml TNFα for 4 h, and the expression of Ccl2 (B) and A20 (C) mRNAs was determined by qRT-PCR. These data represent the average of five independent determinations ± S.D. (error bars). D, the TKD 3T3-L1 cells were stably infected with TWT and TMU lentiviruses as described under “Method details.” The 3T3-L1 TKD, TWT, and TMU preadipocytes were differentiated into adipocytes either left untreated (Basal) or stimulated with 10 ng/ml TNFα for 5 min (TNFα). Cell lysates were prepared and immunoblotted for the indicated proteins. These are representative immunoblots independently performed three times. E and F, the TKD, TWT, and TMU adipocytes were treated with vehicle or 10 ng/ml TNFα for 4 h, and the expression of Ccl2 (E) and A20 (F) mRNAs was determined by qRT-PCR. These data represent the average of three independent determinations ± S.D. *, p < 0.05; ****, p < 0.0001.