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. 2018 Apr 12;293(20):7578–7591. doi: 10.1074/jbc.RA118.002727

Figure 6.

Figure 6.

TIGAR and NEMO compete for HOIP binding. A, HEK293T cells were transfected with the indicated combinations of cDNAs (8 μg total/100-mm dish) for 16 h, followed by immunoblotting of cell lysates for the indicated proteins. Lane 1, 8 μg of empty vector; lane 2, 3 μg of HOIP; lane 3, 2 μg of TIGAR; lane 4, 1 μg of the NEMO; lane 5, 3 μg of HOIP plus 2 μg of TIGAR; lane 6, 3 μg of HOIP plus 1 μg of NEMO; lanes 7–9, 3 μg of HOIP plus 2 μg of TIGAR with increasing amounts of NEMO (0.1, 0.3, 1, and 3 μg, respectively). B, the cell lysates from A were immunoprecipitated (IP) with a FLAG antibody and immunoblotted for the indicated proteins. These are representative immunoblots independently performed two times.