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In vitro aerobactin reconstitution. Shown are overlaid extracted ion chromatograms corresponding to the [M + H]+ masses of metabolites ahLys, citryl-ahLys, and aerobactin from in vitro reaction mixtures containing (A) no enzymes; (B) IucD and IucB; (C) IucD, IucB, and IucA; (D) IucD, IucB, and IucC; or (E) IucA, IucB, IucC, and IucD.
