Table 2. Summarized ranking of the most abundant proteins in Drosophila processing complexes.
Rank | CG | Protein | Detected | ΔAVE (%) |
---|---|---|---|---|
1 | 11 886 | SLBP | 12 | 17.2 |
2 | 12 924 | Lsm11 | 12 | 11.6 |
3 | 9742 | SmG | 10 | 6.4 |
4 | 2097 | Symplekin | 12 | 4.1 |
5 | 1957 | CPSF100 | 12 | 3.6 |
6 | 7698 | CPSF73 | 12 | 2.9 |
7 | 18 591 | SmE | 10 | 2.5 |
8 | 4616 | FLASH | 12 | 2.3 |
9 | 5352 | SmB | 11 | 2.1 |
10 | 7697 | CstF64 | 12 | 1.7 |
11 | 12 938 | Lsm10 | 10 | 1.2 |
12 | 8427 | SmD3 | 10 | 1.1 |
13 | 16 792 | SmF | 8 | 0.7 |
14 | 10 110 | CPSF160 | 11 | 0.8 |
17 | 1109 | WDR33 | 11 | 0.5 |
21 | 1078 | Fip1 | 7 | 0.08 |
24 | 3642 | CPSF30 | 5 | 0.06 |
The hierarchy is based on the value of ΔAVE (%) calculated for all 12 Drosophila experiments (see Supplementary Data for complete data and text for the method of calculation). Of the 12 experiments, 7 were carried out with pcB-dH3/5m and 5 with the dH3 duplex. Omitted from the list are proteins that were competed <50% by SL RNA and anti-U7 oligonucleotide (proteins 15, 18, 22 and 23), and/or were detected in <4 experiments (proteins 16, 19 and 20).