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Defective development of haploid spermatids in Carm1 cKO mice. (A) Periodic acid–Schiff (PAS) staining of the cross-sections of paraffin-embedded testes from WT and cKO mice. Z, Zygotene; P, Pachytene; RS, Round spermatids; ES, Elongating spermatids. (B) Statistic comparison of the average number of spermatids relative to Sertoli cells, calculated from PAS-stained cross-sections. Data were presented as mean ± SEM. *P< 0.05; **P< 0.01 (Student's t test).
