Fig. 3.

Depletion of mTORC1 and, in certain GBM cultures, mTORC2 can promote inhibitory phosphorylation of GSK3B. (A) Western blot of GBM cultures HK157 and HK301 demonstrating efficacy of knockdown and phosphorylation status of GSK3B, ERK, and Akt. (B) Mean relative cell number and standard error bars for a serial dilution of rapamycin treatments in HK157 in the presence or absence of shRICTOR, P = 0.0004 comparing mean IC₅₀ values (N = 3, log scale). (C) Mean relative cell number and standard error bars for a serial dilution of BEZ235 treatments in HK157 in the presence and absence of shRICTOR, P < 0.001 comparing IC₅₀ values (N = 3, log scale). (D) Mean relative cell number and standard error bars for a serial dilution of rapamycin treatments in HK301 in the presence and absence of shRICTOR (P = 0.8618) is depicted comparing IC₅₀ values (N = 2, log scale). (E) Mean relative cell number and standard error bars for a serial dilution of BEZ235 treatments in HK301 in the presence and absence of shRICTOR, P = 0.1012 comparing IC₅₀ values (N = 2, log scale). See also Supplementary Figure S3.