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. 2018 Feb 22;46(9):4699–4714. doi: 10.1093/nar/gky116

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — 23S pre-rRNA is targeted by endo- and exo-nucleolytic pathways for its degradation. Pre-rRNA accumulation was monitored in (A) presence of endogenous Efg1 and compared with cells depleted for Efg1 (B). Yeast cells were grown to mid-log phase in galactose containing media. Glucose was next added within the media and cells were grown in these conditions for 3 h. Aliquots were collected and total RNAs were extracted and subjected to poly(A)+ affinity purification on oligo-dT coated beads. All RNA samples were separated on 1.2% agarose gels, transferred to a nylon membrane and hybridized with specific oligonucleotide probes. Upper panels and mid panels were hybridized with ETS1 (1699) and ITS1 (004) probes, respectively. Lower panels were hybridized with an oligonucleotide specific for PGK1 mRNA (403).