Fig. 3.

CMP5 causes apoptosis in GBMDC and decreases self-renewal capacity in GBMNS. (A) Viability of the indicated primary GBM cells grown as neurospheres or differentiated cells treated with increasing doses of CMP5 for 72 hours was measured by MTT assay. (B) GBMNS and GBMDC treated with Ctrl (DMSO) or CMP5 (25 µM) for 72 hours were analyzed for percentage of cells undergoing apoptosis using annexin V and PI staining. Data shown are percent of annexin-positive cells (±SD) for the indicated GBM cells (**P ≤ 0.001). (C) Increasing number of indicated GBMNS ranging from 10 to 1000 cells per well were seeded and treated with CMP5 (25 µM) or Ctrl (DMSO). Seven days posttreatment, number of neurospheres formed in each well were counted and plotted against the number of cells seeded. The thin black line represents the linear trend. All the experiments were conducted in biological triplicates.