Fig. 4.

CMP5 causes G1 cell cycle arrest in GBMNS. (A) Cell cycle analysis utilizing PI staining of indicated GBMNS and GBMDC treated with DMSO or CMP5 (25 µM) for 24 h posttreatment. Graph represents the percent of cell population in each stage of cell cycle (**P ≤ 0.001). (B) GBMNS-30 transfected with scrambled small interfering (si)RNA (Scr) or PRMT5 siRNA (P5i) were treated with DMSO or CMP5 (25 µM); 72 h posttreatment, cells were subjected to cell cycle analysis and the population of cells in each phase of the cell cycle were quantified. **Statistical significance of P5i + DMSO, Scr + CMP5, and P5i + CMP5 in comparison to control (Scr + DMSO) (**P ≤ 0.001). All the experiments were conducted in 3 biological triplicates.