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Plasmid-probe unbinding initiated by topoisomerase IA. Percentage of pits containing bound complexes over time for a solution containing 752 pM of the 30-base pair probe and 21.1 nM of pUC19 in 20 mM tris, 50 mM potassium acetate, and 100 μg/mL BSA after the addition of 28.48 μg/mL of E. coli topoisomerase IA. Topoisomerase IA was added at t = 0.
