Figure 3.

Differential effects on cell cycles by RSV and CFZ in MM cells. (A), Four MM cell lines LP-1, U266, MM.1S, and MM.1R were treated with RSV (50 μM), CFZ (40 nM), or in a combination of them for 24 h. Cells were harvested and gradually fixed with ethanol for cell cycles analysis through flow cytometery. *P < 0.05; #P < 0.01 compared with the respective control. (B), Four MM cell lines LP-1, U266, MM.1S, and MM.1R were treated the same as in (A). Cell lysates were harvested. Expression of phosphorylated CDK4 and cyclin D1 was measured by Western blotting. Tubulin was used as a loading control.