Figure 5.

Resveratrol increased stress-associated pathways after combination with CFZ. (A), LP-1 and MM.1S cells were treated with RSV (50 μM), CFZ (40 nM) or a combination of them for 24 h. Then, cell lysates were harvested. SIRT1, Smac, Survivin, p53, and phosphorylated p38 were detected through Western blotting. Tubulin was used as a loading control. (B), LP-1 and MM.1S cells were treated with different concentrations (25, 50, 100, μM) of RSV for 24 h. Smac protein levels were detected through Western blotting. Tubulin was used as a loading control. (C), LP-1 cells were transfected with scrambled siRNA and Smac siRNA for 72 h. Then, cells were treated with vehicle control or RSV (50 μM)/CFZ (40 nM) combination for 24 h. Cells lysates were harvested to detect Smac, SIRT1, and survivin expression through Western blotting. Tubulin was used as a loading control. (D), LP-1 and MM.1S cells were transfected with vector control and SIRT1 specific shRNA for 72 h. Then, cells lysates were harvested to detect SIRT1 and survivin expression through Western blotting. Tubulin was used as a loading control.