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. 2018 Apr 6;46(9):4607–4621. doi: 10.1093/nar/gky258

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Distribution on polysome gradients of selected mRNAs identified in the translatome analysis. mRNAs were detected by RT-PCR amplification in each fraction of the polysome gradient from unfertilized eggs (UnF), 1-h post-fertilization embryos (F) or puromycin-treated embryos (F+puro). Amplicons were run on agarose gels and quantified using ImageJ software. Distribution is shown along the gradient as a percentage of total mRNA. Fraction #1 corresponds to the top of the gradient (free mRNAs) and #21 corresponds to the bottom of the gradient. Values are shown as a mean of at least five independent biological replicates, error bars represent SEM (UnF vs F: *P-value < 0.05)