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. 2018 Apr 6;46(9):4607–4621. doi: 10.1093/nar/gky258

Figure 4.

Figure 4.

Impact of mTOR inhibition on mRNA polysomal recruitment. PP242 was added 10 min before fertilization and puromycin 40 min after fertilization, in the indicated samples. Control and treated embryos were harvested at 60 min after fertilization for polysome analysis. mRNAs exhibit mTOR-dependent (A) or mTOR-independent (B) polysomal recruitment. Distribution on polysome gradients was monitored as described in Figure 3 from 1-h post-fertilization embryos (F), in the presence of the PP242 inhibitor (F+PP242) or in the presence of PP242 and puromycin (F+PP242+puro). Values are shown as a mean of at least five independent biological replicates, error bars represent SEM (F vs F+PP242: *P-value < 0.05 and F+PP242 vs F+PP242+puro: †P-value < 0.05) The translation of the mRNAs in the corresponding puromycin-treated embryos (F+puro) is shown in Supplementary Figure S8.