Figure 3.

Detection of antisense RNA and toxin mRNA by northern blot. RNA samples were extracted from 630Δerm strain grown at exponential phase (E, 4 h of growth), late exponential phase (LE, 6 h of growth), entry to stationary phase (S, 10 h of growth) or under nutrient starvation conditions (St). 5S RNA at the bottom serves as loading control. The arrows show the detected transcripts with their size estimated by comparison with RNA molecular weight standards. As indicated at the top, the blots were hybridized either with antitoxin- or toxin-specific probe. The same 5S control panel is shown when reprobing of the same membrane was performed.