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. 2018 Feb 26;46(9):4733–4751. doi: 10.1093/nar/gky124

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — Effect of short and full-length antitoxin co-overexpression with native toxin in trans for CD2517.1-RCd8 and CD2907.1-RCd9/CD0956.2-RCd10 TA modules on growth in solid (A and B) and liquid (C and D) medium. (A) Growth of C. difficile strains CDIP1191 (630/pCD2517.1-RCd8), CDIP357 (630/pCD2517.1), CDIP1161 (630/pCD2517.1 and long RCd8), CDIP1130 (630/pCD2517.1 and short RCd8), CDIP1162 (630/pCD2517.1 and long RCd9) and CDIP1131 (630/pCD2517.1 and short RCd9) on BHI agar plates supplemented with Tm and 2.5 ng/ml of ATc inducer after 24 h of incubation at 37°C. (B) Growth of C. difficile strains CDIP1192 (630/pCD2907.1-RCd9), CDIP317 (630/pCD2907.1), CDIP1164 (630/pCD2907.1 and long RCd9), CDIP1133 (630/pCD2907.1 and short RCd9), CDIP1163 (630/pCD2907.1 and long RCd8) and CDIP1132 (630/pCD2907.1 and short RCd8) on BHI agar plates supplemented with Tm and 3 ng/ml of ATc inducer after 24 h of incubation at 37°C. (C) Growth of the same C. difficile strains as in (A) in TY medium at 37°C in the presence of 2.5 ng/ml ATc using a GloMax plate reader (Promega). The mean values are shown for three independent experiments. (D) Growth of the same C. difficile strains as in (B) in TY medium at 37°C in the presence of 3 ng/ml ATc using a GloMax plate reader (Promega). The mean values are shown for three independent experiments.