Table 2. Differential expression of TA and CRISPR-Cas systems revealed by transcriptome and/or qRT-PCR analysis.
| Gene ID | Function | Ratio sigB/630Δerm Microarraya | Ratio sigB/630Δerm qRT-PCR | Ratio biofilm/plankton qRT-PCR |
|---|---|---|---|---|
| CD2982 b | CRISPR-associated Cas6 family protein | 0.19 | 0.22 | 14.7 |
| CD2981 | CRISPR-associated protein, CXXC-CXXC | 0.21 | ||
| CD2980 | CRISPR-associated autoregulator DevR family protein | 0.26 | ||
| CD2979 | CRISPR-associated Cas5 family protein | 0.25 | ||
| CD2978 | CRISPR-associated Cas3 family helicase | 0.30 | ||
| CD2977 | CRISPR-associated Cas4 family protein | 0.33 | ||
| CD2976 | CRISPR-associated Cas1 family protein | 0.37 | ||
| CD2975 | CRISPR-associated Cas2 family protein | 0.37 | ||
| CD2455 c | CRISPR-associated protein | 0.55 | 0.61 | 9.4 |
| CD2454 | Conserved hypothetical protein | 0.55 | ||
| CD2453 | CRISPR-associated negative autoregulator | 0.47 | ||
| CD2452 | CRISPR-associated protein | 0.53 | ||
| CD1233.1 | Toxin of TA associated with CRISPR 6 | 0.51 | ||
| CD2517.1 | Toxin of TA associated with CRISPR 12 | 0.25 | 0.42 | 26.0 |
| RCd8 | Antitoxin of TA associated with CRISPR 12 | 0.67 | 7.3 | |
| CD630_n00860 | CRISPR 12 | 7.7 | ||
| CD2907.1 | Toxin of TA associated with CRISPR 16/15 | 1.8 | ||
| RCd9/RCd10 | Antitoxin of TA associated with CRISPR 16/15 /CRISPR 3/4 | 0.5 | 0.42 | 2.3 |
| CD630_n00990 | CRISPR 16/15 | 0.54 | 9.8 |
Gene names and functions correspond to those indicated in the MaGe database Clostriscope (https://www.genoscope.cns.fr).
aA gene was considered as differentially expressed between the strain 630Δerm and the sigB mutant when the P-value is < 0.05.
bFirst gene of the complete cas operon CD2982-CD2975.
cFirst gene of the partial cas operon CD2455-CD2452. SQ1781 corresponds to RCd8, CD630_n01000 to RCd9 and CD630_n00370 to RCd10.