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. 2018 Apr 19;46(9):4354–4369. doi: 10.1093/nar/gky286

Figure 5.

Figure 5.

Validation of tumor-suppressor PCI interactions. (A) Perturbations using PTEN 3′ UTR and cDNA in PC3, where PTEN is –/–, suggest that RB1 and TP53 regulation by the PTEN 3′ UTR is independent of PTEN protein expression. (B) Silencing RB1 and transfecting its 3′ UTR in U2-OS cells down and upregulated PTEN and TP53 mRNA expression, respectively. Transfecting RB1 3′ UTR rescued the effects of siRB1 on PTEN and TP53 mRNA expression (P < 0.1 for PTEN and P < 1E–4 for TP53). Negative controls include perturbations of MAPK13, RSAD1 and THNSL1. (C) siRNA-mediated silencing of RB1 in U2-OS, SK-MEL-28 and A549 reduced PTEN and TP53 luciferase activity. Data are represented as mean ± SEM.