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. 2018 Mar 5;46(9):e57. doi: 10.1093/nar/gky152

Figure 4.

Figure 4.

Role of the AAUAAA polyA motif in gRNA production and packaging. (A) 5′PolyA mutants contain point mutations or deletions to the AAUAAA sequence. SL2 mutant containing substitutions within the U1snRNA binding site. (B) Production of gRNA and spliced viral RNA (mRNA Tat) for 5′ polyA and SL2 mutants. Bar charts represent six independent experiments. (C) Relative packaging efficiency of gRNA and spliced viral RNA into viral particles, expressed as a virus/cellular RNA (36). Bar charts represent 3 independent experiments. Statistical tests were carried out using ANOVA corrected for multiple comparisons.