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Effects of single base changes in 40 bp λPR ITS on promoter escape in a context of λPR promoter (A), deoB promoter (B) and UV5 promoter (C). Wt sequence is shown in green. Examples of promoter context dependence of base changes at a specific position (+2, D–F; +12, G). Examples of significant effects of base changes on promoter escape at positions far away from +1 (+18, H; +35, I). Bar colours in panels D–I depict identity of the base: green—A; yellow—G; red—T; blue—C.
