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. 2018 Feb 27;46(9):4487–4504. doi: 10.1093/nar/gky155

Figure 8.

Figure 8.

ATF7 mediates telomere shortening not by regulating H3K9me3 level. (A, B) ATF7 mediates a TNF-α–induced decrease in histone H3K9me3 levels on telomeres. WT or Atf7–/– MEFs (A), or HeLa cells (B), were treated with TNF-α for the indicated times, and ChIP/slot-blot hybridization was performed using anti-histone H3 and anti-H3K9me3 antibodies with the telomere probe. Average H3K9me3/H3 ratios are shown ± SD (n = 3), and typical slot-blot hybridization data are shown below. *P < 0.05; NS, not significant. (C) Interaction between ATF7 and Suv39h1 is not abrogated by TNF-α treatment. HeLa cells expressing Flag-ATF7 were treated with TNF-α (100 ng/ml) for the indicated times; whole-cell lysates were prepared and immunoprecipitated with anti-ATF7; and the immunoprecipitates were immunoblotted with anti-Suv39h1 antibody. (D) Release of Suv39h1 from telomere following TNF-α treatment. WT or Atf7–/– MEFs were treated with TNF-α (100 ng/ml) for the indicated times, and then subjected to ChIP/slot-blot hybridization using anti-Suv39h1 antibodies and telomeric probes. Average signals ± SD (n = 3) are shown below. *P < 0.05; **P < 0.01; NS, not significant.